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Rapid sample preparation technique for the determination of pyrrolizidine alkaloids in plant extracts

Tipo de material: TextoTextoSeries ; Journal of Chromatography A, 668(2), p.427-433, 1994Trabajos contenidos:
  • Chizzola, R
Recursos en línea: Resumen: To determine toxic pyrrolizidine alkaloids from various plant sources, a sample preparation technique is described that affords alkaloid fractions suitable for capillary GC or TLC determination. The procedure includes the reduction of the alkaloid N-oxides with the oxygen-absorbing resin Serdoxit and a clean-up with strong cation-exchange solid-phase columns. If desired an enrichment of the alkaloids can be obtained. The method was tested with methanolic extracts from Senecio vulgaris, Petasites hybridus and Symphytum ofjcinale.
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To determine toxic pyrrolizidine alkaloids from various plant sources, a sample preparation technique is described that affords alkaloid fractions suitable for capillary GC or TLC determination. The procedure includes the reduction of the alkaloid N-oxides with the oxygen-absorbing resin Serdoxit and a clean-up with strong cation-exchange solid-phase columns. If desired an enrichment of the alkaloids can be obtained. The method was tested with methanolic extracts from Senecio vulgaris, Petasites hybridus and Symphytum ofjcinale.

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