The Isolation of FOS-1, a Gene Encoding a Putative Two-Component Histidine Kinase from Aspergillus fumigatus
The Isolation of FOS-1, a Gene Encoding a Putative Two-Component Histidine Kinase from Aspergillus fumigatus
- Fungal Genetics and Biology , 31(1), p.55-67, 2000 .
In fungi, two-component histidine kinases are involved in response mechanisms to extracellular changes in osmolarity, resistance to dicarboximide fungicides, and cell-wall assembly. In the human opportunistic fungus, Candida albicans, each of the three histidine kinases plays a role in virulence. Here, we identify, for the first time, a gene, FOS-1, from the human pathogenic fungus Aspergillus fumigatus that predicts a protein with homology to two-component histidine kinases. The predicted FOS-1 protein is highly homologous to bacterial and other fungal histidine kinases in several functional domains, but is divergent at the amino- and carboxy-termini. A mutant lacking the FOS-1 locus, ?FOS-1, did not exhibit a detectable defect in either hyphal growth or morphology when grown on solid or liquid medium. However, in liquid medium, conidiophore development of the ?FOS-1 mutant was delayed. Compared to wild type, the ?FOS-1 strain was neither osmotically sensitive nor sensitive or resistant to a number of nondicarboximide antifungal drugs, but was highly resistant to dicarboximide fungicides and resistant to novozym 234, suggesting that FOS-1p may play a role in the regulation of cell-wall assembly.
ASPERGILLUS FUMIGATUS
TWO-COMPONENT HISTIDINE KINASE
DICARBOXIMIDE ANTIFUNGAL COMPOUNDS
FOS-1
CELL-WALL ASSEMBLY
In fungi, two-component histidine kinases are involved in response mechanisms to extracellular changes in osmolarity, resistance to dicarboximide fungicides, and cell-wall assembly. In the human opportunistic fungus, Candida albicans, each of the three histidine kinases plays a role in virulence. Here, we identify, for the first time, a gene, FOS-1, from the human pathogenic fungus Aspergillus fumigatus that predicts a protein with homology to two-component histidine kinases. The predicted FOS-1 protein is highly homologous to bacterial and other fungal histidine kinases in several functional domains, but is divergent at the amino- and carboxy-termini. A mutant lacking the FOS-1 locus, ?FOS-1, did not exhibit a detectable defect in either hyphal growth or morphology when grown on solid or liquid medium. However, in liquid medium, conidiophore development of the ?FOS-1 mutant was delayed. Compared to wild type, the ?FOS-1 strain was neither osmotically sensitive nor sensitive or resistant to a number of nondicarboximide antifungal drugs, but was highly resistant to dicarboximide fungicides and resistant to novozym 234, suggesting that FOS-1p may play a role in the regulation of cell-wall assembly.
ASPERGILLUS FUMIGATUS
TWO-COMPONENT HISTIDINE KINASE
DICARBOXIMIDE ANTIFUNGAL COMPOUNDS
FOS-1
CELL-WALL ASSEMBLY