Molecular marker assisted transfer of resistance to TSWV in tomato elite lines
Molecular marker assisted transfer of resistance to TSWV in tomato elite lines
- The Journal of Horticultural Science and Biotechnology,, 79(5), p.806-810, 2004 .
backcross breeding programme was performed to transfer the Sw-5 gene conferring resistance to Tomato Spotted Wilt Virus (TSWV)to a fresh market (Poly 39)and a processing (AD-17)line of tomato, previously selected for good agronomic traits. In both cases, F1BC5, F2BC5, F3BC5 were obtained from F1 crosses with the donor parent Stevens, an Sw-5 resistant variety. The backcross schemes were assisted by a CAPS (cleaved amplified polymorphic sequence)marker tightly linked to the resistant gene; the codominant nature of this marker was particularly useful to select resistant heterozygous/homozygous plants. Further, an inoculation test with a TSWV virus strain was also performed to confirm the successful introgression of resistance in both elite lines. The advantages of using MAS for disease resistance are also discussed.
backcross breeding programme was performed to transfer the Sw-5 gene conferring resistance to Tomato Spotted Wilt Virus (TSWV)to a fresh market (Poly 39)and a processing (AD-17)line of tomato, previously selected for good agronomic traits. In both cases, F1BC5, F2BC5, F3BC5 were obtained from F1 crosses with the donor parent Stevens, an Sw-5 resistant variety. The backcross schemes were assisted by a CAPS (cleaved amplified polymorphic sequence)marker tightly linked to the resistant gene; the codominant nature of this marker was particularly useful to select resistant heterozygous/homozygous plants. Further, an inoculation test with a TSWV virus strain was also performed to confirm the successful introgression of resistance in both elite lines. The advantages of using MAS for disease resistance are also discussed.