Molecular marker assisted transfer of resistance to TSWV in tomato elite lines
Tipo de material:
TextoSeries ; The Journal of Horticultural Science and Biotechnology,, 79(5), p.806-810, 2004Trabajos contenidos: - LANGELLA, R
- ERCOLANO, M.R
- MONTI, L.- M
- FRUSCIANTE, L
- BARONE, A
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backcross breeding programme was performed to transfer the Sw-5 gene conferring resistance to Tomato Spotted Wilt Virus (TSWV)to a fresh market (Poly 39)and a processing (AD-17)line of tomato, previously selected for good agronomic traits. In both cases, F1BC5, F2BC5, F3BC5 were obtained from F1 crosses with the donor parent Stevens, an Sw-5 resistant variety. The backcross schemes were assisted by a CAPS (cleaved amplified polymorphic sequence)marker tightly linked to the resistant gene; the codominant nature of this marker was particularly useful to select resistant heterozygous/homozygous plants. Further, an inoculation test with a TSWV virus strain was also performed to confirm the successful introgression of resistance in both elite lines. The advantages of using MAS for disease resistance are also discussed.
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