Cloning and heterologous expression of a cDNA encoding l-deoxy-D-xylulose-5-phosphate reductoisomerase of Arabidopsis thaliana1
Cloning and heterologous expression of a cDNA encoding l-deoxy-D-xylulose-5-phosphate reductoisomerase of Arabidopsis thaliana1
- FEBS Letters, 455, p.140-144, 1999 .
Various plant isoprenoids are synthesized via the nonmevalonate pathway of isopentenyl diphosphate formation. In this pathway, 1-deoxy-D-xylulose 5-phosphate (DOXP), the first intermediate, is transformed to 2-C-methyl-D-erythritol 4-phosphate (MEP)by an enzyme which was recently cloned from Escherichia coli. In order to find a plant homologue of this 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR)we cloned a cDNA fragment from Arabidopsis thaliana which has high homology to the E. coli DXR. By expression of this fragment in E. coli we could demonstrate that it encodes a protein which transforms DOXP to MEP. The antibiotic fosmidomycin pecifically inhibits this DXR enzyme activity.
1-DEOXY-D-XYLULOSE
1-DEOXY-D-XYLULOSE 5-PHOSPHATE REDUCTOISOMERASE
1-DEOXY-D-XYLULOSE 5-PHOSPHATE PATHWAY
ISOPRENOID BIOSYNTHESIS
2-C-METHYL-D-ERYTHRITOL
Various plant isoprenoids are synthesized via the nonmevalonate pathway of isopentenyl diphosphate formation. In this pathway, 1-deoxy-D-xylulose 5-phosphate (DOXP), the first intermediate, is transformed to 2-C-methyl-D-erythritol 4-phosphate (MEP)by an enzyme which was recently cloned from Escherichia coli. In order to find a plant homologue of this 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR)we cloned a cDNA fragment from Arabidopsis thaliana which has high homology to the E. coli DXR. By expression of this fragment in E. coli we could demonstrate that it encodes a protein which transforms DOXP to MEP. The antibiotic fosmidomycin pecifically inhibits this DXR enzyme activity.
1-DEOXY-D-XYLULOSE
1-DEOXY-D-XYLULOSE 5-PHOSPHATE REDUCTOISOMERASE
1-DEOXY-D-XYLULOSE 5-PHOSPHATE PATHWAY
ISOPRENOID BIOSYNTHESIS
2-C-METHYL-D-ERYTHRITOL