Cloning and heterologous expression of a cDNA encoding l-deoxy-D-xylulose-5-phosphate reductoisomerase of Arabidopsis thaliana1 (Record no. 41123)

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fixed length control field 01661nam a2200265Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625124649.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-6784
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245 10 - TITLE STATEMENT
Title Cloning and heterologous expression of a cDNA encoding l-deoxy-D-xylulose-5-phosphate reductoisomerase of Arabidopsis thaliana1
490 0# - SERIES STATEMENT
Volume/sequential designation FEBS Letters, 455, p.140-144, 1999
520 3# - SUMMARY, ETC.
Summary, etc. Various plant isoprenoids are synthesized via the nonmevalonate pathway of isopentenyl diphosphate formation. In this pathway, 1-deoxy-D-xylulose 5-phosphate (DOXP), the first intermediate, is transformed to 2-C-methyl-D-erythritol 4-phosphate (MEP)by an enzyme which was recently cloned from Escherichia coli. In order to find a plant homologue of this 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR)we cloned a cDNA fragment from Arabidopsis thaliana which has high homology to the E. coli DXR. By expression of this fragment in E. coli we could demonstrate that it encodes a protein which transforms DOXP to MEP. The antibiotic fosmidomycin pecifically inhibits this DXR enzyme activity.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 1-DEOXY-D-XYLULOSE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 1-DEOXY-D-XYLULOSE 5-PHOSPHATE REDUCTOISOMERASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 1-DEOXY-D-XYLULOSE 5-PHOSPHATE PATHWAY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ISOPRENOID BIOSYNTHESIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 2-C-METHYL-D-ERYTHRITOL
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Schwender, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Muller, Ch.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zeidler, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lichtenthaler, H.K.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/17fLBAMxYhkDtTXhu0XupuduP3K_OKVMl/view?usp=drivesdk">https://drive.google.com/file/d/17fLBAMxYhkDtTXhu0XupuduP3K_OKVMl/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-6784 25.06.2025 25.06.2025 Documentos solicitados