Identification of Isoquinoline Alkaloids in Crude Extracts by High Temperature Gas Chromatography-Mass Spectrometry
Identification of Isoquinoline Alkaloids in Crude Extracts by High Temperature Gas Chromatography-Mass Spectrometry
- PhytoChemical Analysis, 10(5), p.254-258, 1999 .
Crude ethanol extracts of leaves of Croton (Euphorbiaceae)species (C. hemiargyreus var. gymnodiscus, C. hemiargyreus var. hemiargyreus and C. echinocarpus)were analysed using on-column injection high temperature gas chromatography coupled with mass spectrometry. Several isoquinoline alkaloids were identified, including isocorydine, corydine, glaucine and laurelliptine as the principal constituents. The cold on-column technique proved to be appropriate for injection of these compounds. The technique is shown to be a valuable tool and an alternative technique to classical phytochemical procedures permitting the fast analysis of alkaloid mixtures without the need for (extensive)fractionation and derivatisation.
COLD ON-COLUMN INJECTOR
HIGH TEMPERATURE GAS CHROMATOGRAPHY
ALKALOIDS
EUPHORBIACEAE
CROTON
Crude ethanol extracts of leaves of Croton (Euphorbiaceae)species (C. hemiargyreus var. gymnodiscus, C. hemiargyreus var. hemiargyreus and C. echinocarpus)were analysed using on-column injection high temperature gas chromatography coupled with mass spectrometry. Several isoquinoline alkaloids were identified, including isocorydine, corydine, glaucine and laurelliptine as the principal constituents. The cold on-column technique proved to be appropriate for injection of these compounds. The technique is shown to be a valuable tool and an alternative technique to classical phytochemical procedures permitting the fast analysis of alkaloid mixtures without the need for (extensive)fractionation and derivatisation.
COLD ON-COLUMN INJECTOR
HIGH TEMPERATURE GAS CHROMATOGRAPHY
ALKALOIDS
EUPHORBIACEAE
CROTON