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Identification of Isoquinoline Alkaloids in Crude Extracts by High Temperature Gas Chromatography-Mass Spectrometry

Tipo de material: TextoTextoSeries ; PhytoChemical Analysis, 10(5), p.254-258, 1999Trabajos contenidos:
  • Pereira, A.S
  • Amaral, A.C.F
  • Barnes, R.A
  • Cardoso, J.N
  • Aquino Neto, F.R
Tema(s): Recursos en línea: Resumen: Crude ethanol extracts of leaves of Croton (Euphorbiaceae)species (C. hemiargyreus var. gymnodiscus, C. hemiargyreus var. hemiargyreus and C. echinocarpus)were analysed using on-column injection high temperature gas chromatography coupled with mass spectrometry. Several isoquinoline alkaloids were identified, including isocorydine, corydine, glaucine and laurelliptine as the principal constituents. The cold on-column technique proved to be appropriate for injection of these compounds. The technique is shown to be a valuable tool and an alternative technique to classical phytochemical procedures permitting the fast analysis of alkaloid mixtures without the need for (extensive)fractionation and derivatisation.
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Crude ethanol extracts of leaves of Croton (Euphorbiaceae)species (C. hemiargyreus var. gymnodiscus, C. hemiargyreus var. hemiargyreus and C. echinocarpus)were analysed using on-column injection high temperature gas chromatography coupled with mass spectrometry. Several isoquinoline alkaloids were identified, including isocorydine, corydine, glaucine and laurelliptine as the principal constituents. The cold on-column technique proved to be appropriate for injection of these compounds. The technique is shown to be a valuable tool and an alternative technique to classical phytochemical procedures permitting the fast analysis of alkaloid mixtures without the need for (extensive)fractionation and derivatisation.

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