Dye-labelled substrates for the assay and detection of chitinase and lysozyme activity
Dye-labelled substrates for the assay and detection of chitinase and lysozyme activity
- Journal of MicroBiological Methods, 12, p.197-205, 1990 .
A carboxymethyl-substituted soluble chitin and a reprecipitated colloidalchitin derivative were covalently linked with Remazol Brilliant Violet 5R. Both conjugates are new dye-labelled substrates suitable for the assay and detection of chitinase and lysozyme activity. A reliable colorimetric microassay adapted to microtitre plates was established, based on the precipitability of carboxymethyl-chitin-Remazol Brilliant Violet 5R from buffered solutions with hydrochloric acid. Furthermore, sensitive plate-clearing assays were developed for the isolation and detection of chitinolytic microbial populations, and for the screening of pure cultures.
CHITINASE ASSAY
CHITIN DERIVATIVE
PLATE-CLEARING ASSAY
REMAZOL BRILLIANT VIOLET 5R
A carboxymethyl-substituted soluble chitin and a reprecipitated colloidalchitin derivative were covalently linked with Remazol Brilliant Violet 5R. Both conjugates are new dye-labelled substrates suitable for the assay and detection of chitinase and lysozyme activity. A reliable colorimetric microassay adapted to microtitre plates was established, based on the precipitability of carboxymethyl-chitin-Remazol Brilliant Violet 5R from buffered solutions with hydrochloric acid. Furthermore, sensitive plate-clearing assays were developed for the isolation and detection of chitinolytic microbial populations, and for the screening of pure cultures.
CHITINASE ASSAY
CHITIN DERIVATIVE
PLATE-CLEARING ASSAY
REMAZOL BRILLIANT VIOLET 5R