Dye-labelled substrates for the assay and detection of chitinase and lysozyme activity (Record no. 42468)

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control field MX-MdCICY
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control field 20250625124715.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-8161
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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245 10 - TITLE STATEMENT
Title Dye-labelled substrates for the assay and detection of chitinase and lysozyme activity
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of MicroBiological Methods, 12, p.197-205, 1990
520 3# - SUMMARY, ETC.
Summary, etc. A carboxymethyl-substituted soluble chitin and a reprecipitated colloidalchitin derivative were covalently linked with Remazol Brilliant Violet 5R. Both conjugates are new dye-labelled substrates suitable for the assay and detection of chitinase and lysozyme activity. A reliable colorimetric microassay adapted to microtitre plates was established, based on the precipitability of carboxymethyl-chitin-Remazol Brilliant Violet 5R from buffered solutions with hydrochloric acid. Furthermore, sensitive plate-clearing assays were developed for the isolation and detection of chitinolytic microbial populations, and for the screening of pure cultures.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHITINASE ASSAY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHITIN DERIVATIVE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLATE-CLEARING ASSAY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element REMAZOL BRILLIANT VIOLET 5R
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wirth, S.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Wolf, G.A.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1PMNqjdYjnoNc741w-ZXMiJQ1v6hWGB3G/view?usp=drivesdk">https://drive.google.com/file/d/1PMNqjdYjnoNc741w-ZXMiJQ1v6hWGB3G/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-8161 25.06.2025 25.06.2025 Documentos solicitados