Selective Solvent Extraction of Ruberythric Acid from Madder Roots and Subsequent Hydrolysis with Beta-Glucosidase
Selective Solvent Extraction of Ruberythric Acid from Madder Roots and Subsequent Hydrolysis with Beta-Glucosidase
- Journal of Fermentation and BioEngineering, 8186), p.567-569, 1996 .
Selective solvent extraction of anthraquinones (alizarin and lucidin)and their prlmeverosides (ruherythric acid and lucidin-3-o-primeveroside)from madder roots could be achieved using two-phase extractm with chloroform (CHCl& and potassium hydroxide aqueous solution (KOH,). Ruberythrlc acid extracted to the aqueous phase could be selectively and completely converted to aliznrln within 360 mln by enzymatic hydrolysis with B-glucosidase at 50°C and at pH 5, reducing the inhibitory effect on the reaction caused by alizarin, which is not extracted in appreciable amounts into the aqueous phase, without appreciable hydrolysis of ldcidln-3- o-primeveroside. It was also found that ruberythrlc acid extracted from madder hairy roots by CHC13- KOH,, two-phase extraction was efficiently converted to alizarin in the same manner.
SOLVENT EXTRACTION
RUBERYTHRIC ACID
MADDER ROOT
ENZYMATIC HYDROLYSIS
Selective solvent extraction of anthraquinones (alizarin and lucidin)and their prlmeverosides (ruherythric acid and lucidin-3-o-primeveroside)from madder roots could be achieved using two-phase extractm with chloroform (CHCl& and potassium hydroxide aqueous solution (KOH,). Ruberythrlc acid extracted to the aqueous phase could be selectively and completely converted to aliznrln within 360 mln by enzymatic hydrolysis with B-glucosidase at 50°C and at pH 5, reducing the inhibitory effect on the reaction caused by alizarin, which is not extracted in appreciable amounts into the aqueous phase, without appreciable hydrolysis of ldcidln-3- o-primeveroside. It was also found that ruberythrlc acid extracted from madder hairy roots by CHC13- KOH,, two-phase extraction was efficiently converted to alizarin in the same manner.
SOLVENT EXTRACTION
RUBERYTHRIC ACID
MADDER ROOT
ENZYMATIC HYDROLYSIS