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Selective Solvent Extraction of Ruberythric Acid from Madder Roots and Subsequent Hydrolysis with Beta-Glucosidase

Tipo de material: TextoTextoSeries ; Journal of Fermentation and BioEngineering, 8186), p.567-569, 1996Trabajos contenidos:
  • Masawaki, T
  • Taya, M
  • Tone, S
Tema(s): Recursos en línea: Resumen: Selective solvent extraction of anthraquinones (alizarin and lucidin)and their prlmeverosides (ruherythric acid and lucidin-3-o-primeveroside)from madder roots could be achieved using two-phase extractm with chloroform (CHCl& and potassium hydroxide aqueous solution (KOH,). Ruberythrlc acid extracted to the aqueous phase could be selectively and completely converted to aliznrln within 360 mln by enzymatic hydrolysis with B-glucosidase at 50°C and at pH 5, reducing the inhibitory effect on the reaction caused by alizarin, which is not extracted in appreciable amounts into the aqueous phase, without appreciable hydrolysis of ldcidln-3- o-primeveroside. It was also found that ruberythrlc acid extracted from madder hairy roots by CHC13- KOH,, two-phase extraction was efficiently converted to alizarin in the same manner.
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Selective solvent extraction of anthraquinones (alizarin and lucidin)and their prlmeverosides (ruherythric acid and lucidin-3-o-primeveroside)from madder roots could be achieved using two-phase extractm with chloroform (CHCl& and potassium hydroxide aqueous solution (KOH,). Ruberythrlc acid extracted to the aqueous phase could be selectively and completely converted to aliznrln within 360 mln by enzymatic hydrolysis with B-glucosidase at 50°C and at pH 5, reducing the inhibitory effect on the reaction caused by alizarin, which is not extracted in appreciable amounts into the aqueous phase, without appreciable hydrolysis of ldcidln-3- o-primeveroside. It was also found that ruberythrlc acid extracted from madder hairy roots by CHC13- KOH,, two-phase extraction was efficiently converted to alizarin in the same manner.

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