Purification and identification of Capulin (Prunus serotina Ehrh)anthocyanins
Purification and identification of Capulin (Prunus serotina Ehrh)anthocyanins
- Food Chemistry, 65(2), p.201-206, 1999 .
Capulin (Prunus serotina Ehrh)anthocyanins were extracted from liquid nitrogen powdered epidermal tissue using acetone, the aqueous acetone extract partitioned with chloroform and puriRed in a C-18 solid-phase cartridge. pH-di_erential and bisulRte bleaching methods were used to determine monomeric anthocyanin content and polymeric color. Pigments were identiRed by spectral analyses, HPLC and mass spectroscopy. Information from HPLC proRles, saponiRcation and acid hydrolysis of the capulin anthocyanins showed that the two major pigments were cyanidin-3-glucoside (34
Capulin (Prunus serotina Ehrh)anthocyanins were extracted from liquid nitrogen powdered epidermal tissue using acetone, the aqueous acetone extract partitioned with chloroform and puriRed in a C-18 solid-phase cartridge. pH-di_erential and bisulRte bleaching methods were used to determine monomeric anthocyanin content and polymeric color. Pigments were identiRed by spectral analyses, HPLC and mass spectroscopy. Information from HPLC proRles, saponiRcation and acid hydrolysis of the capulin anthocyanins showed that the two major pigments were cyanidin-3-glucoside (34