Electroporation of protoplasts
Electroporation of protoplasts
- Journal of Tissue Culture Methods, 12(4), p.121-126, 1989 .
A protocol is described for the delivery- of plasmid DNA into plant protoplasts by means of electroporation. Expression of the DNA is monitored 24 h later by a "transient assay" for chloramphenicol acetyltransferase (CATL Stable transformants are recovered by selecting for kanamycin resistance using a modified version of the agarose-bead technique.
DIRECT GENE TRANSFER
STABLE TRANSFORMATION
DNA UPTAKE
TRANSIENT EXPRESSION
CAT EXPRESSION
KANAMYCIN RESISTANCE
A protocol is described for the delivery- of plasmid DNA into plant protoplasts by means of electroporation. Expression of the DNA is monitored 24 h later by a "transient assay" for chloramphenicol acetyltransferase (CATL Stable transformants are recovered by selecting for kanamycin resistance using a modified version of the agarose-bead technique.
DIRECT GENE TRANSFER
STABLE TRANSFORMATION
DNA UPTAKE
TRANSIENT EXPRESSION
CAT EXPRESSION
KANAMYCIN RESISTANCE