Image from Google Jackets

Electroporation of protoplasts

Tipo de material: TextoTextoSeries ; Journal of Tissue Culture Methods, 12(4), p.121-126, 1989Trabajos contenidos:
  • Bates, G.W
Tema(s): Recursos en línea: Resumen: A protocol is described for the delivery- of plasmid DNA into plant protoplasts by means of electroporation. Expression of the DNA is monitored 24 h later by a "transient assay" for chloramphenicol acetyltransferase (CATL Stable transformants are recovered by selecting for kanamycin resistance using a modified version of the agarose-bead technique.
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Collection Call number Status Date due Barcode
Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-9940 (Browse shelf(Opens below)) Available

A protocol is described for the delivery- of plasmid DNA into plant protoplasts by means of electroporation. Expression of the DNA is monitored 24 h later by a "transient assay" for chloramphenicol acetyltransferase (CATL Stable transformants are recovered by selecting for kanamycin resistance using a modified version of the agarose-bead technique.

There are no comments on this title.

to post a comment.