IHneclreenaese Vdi goeilo alaccs uemt aull.ation in seeds Increasing seed oil content in oil-seed rape ( Brassica napus L.)by over-expression of a yeast glycerol-3-phosphate dehydrogenase under the control of a seed-specific promoter
IHneclreenaese Vdi goeilo alaccs uemt aull.ation in seeds Increasing seed oil content in oil-seed rape ( Brassica napus L.)by over-expression of a yeast glycerol-3-phosphate dehydrogenase under the control of a seed-specific promoter
- Plant Biotechnology Journal, 5(3), p.431-441, 2007 .
Previous attempts to manipulate oil synthesis in plants have mainly concentrated on the genes involved in the biosynthesis and use of fatty acids, neglecting the possible role of glycerol-3-phosphate supply on the rate of triacylglycerol synthesis. In this study, a yeast gene coding for cytosolic glycerol-3-phosphate dehydrogenase ( gpd 1)was expressed in transgenic oil-seed rape under the control of the seed-specific napin promoter. It was found that a twofold increase in glycerol-3-phosphate dehydrogenase activity led to a three- to fourfold increase in the level of glycerol-3-phosphate in developing seeds, resulting in a 40
BIOFUEL
OIL CONTENT
TRANSGENIC CROPS
METABOLIC REGULATION
Previous attempts to manipulate oil synthesis in plants have mainly concentrated on the genes involved in the biosynthesis and use of fatty acids, neglecting the possible role of glycerol-3-phosphate supply on the rate of triacylglycerol synthesis. In this study, a yeast gene coding for cytosolic glycerol-3-phosphate dehydrogenase ( gpd 1)was expressed in transgenic oil-seed rape under the control of the seed-specific napin promoter. It was found that a twofold increase in glycerol-3-phosphate dehydrogenase activity led to a three- to fourfold increase in the level of glycerol-3-phosphate in developing seeds, resulting in a 40
BIOFUEL
OIL CONTENT
TRANSGENIC CROPS
METABOLIC REGULATION