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IHneclreenaese Vdi goeilo alaccs uemt aull.ation in seeds Increasing seed oil content in oil-seed rape ( Brassica napus L.)by over-expression of a yeast glycerol-3-phosphate dehydrogenase under the control of a seed-specific promoter

Tipo de material: TextoTextoSeries ; Plant Biotechnology Journal, 5(3), p.431-441, 2007Trabajos contenidos:
  • Vigeolas, H
  • Waldeck, P
  • Zank, T
  • Geigenberger, P
Tema(s): Recursos en línea: Resumen: Previous attempts to manipulate oil synthesis in plants have mainly concentrated on the genes involved in the biosynthesis and use of fatty acids, neglecting the possible role of glycerol-3-phosphate supply on the rate of triacylglycerol synthesis. In this study, a yeast gene coding for cytosolic glycerol-3-phosphate dehydrogenase ( gpd 1)was expressed in transgenic oil-seed rape under the control of the seed-specific napin promoter. It was found that a twofold increase in glycerol-3-phosphate dehydrogenase activity led to a three- to fourfold increase in the level of glycerol-3-phosphate in developing seeds, resulting in a 40
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Previous attempts to manipulate oil synthesis in plants have mainly concentrated on the genes involved in the biosynthesis and use of fatty acids, neglecting the possible role of glycerol-3-phosphate supply on the rate of triacylglycerol synthesis. In this study, a yeast gene coding for cytosolic glycerol-3-phosphate dehydrogenase ( gpd 1)was expressed in transgenic oil-seed rape under the control of the seed-specific napin promoter. It was found that a twofold increase in glycerol-3-phosphate dehydrogenase activity led to a three- to fourfold increase in the level of glycerol-3-phosphate in developing seeds, resulting in a 40

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