High-Level Transient Expression of Recombinant Protein in Lettuce
High-Level Transient Expression of Recombinant Protein in Lettuce
- Biotechnology and BioEngineering, 91(7), p.861-671, 2005 .
Transient expression following agroinfiltration of plant tissue was investigated as a system for producing recombinant protein. As a model system, Agrobacterium tumefaciens containing the b-glucuronidase (GUS)gene was vacuum infiltrated into lettuce leaf disks. Infiltration with a suspension of 109 colony forming units/mL followed by incubation for 72 h at 228C in continuous darkness produced a maximum of 0.16
AGROINFILTRATION
LETTUCE
AGROBACTERIUM
BGLUCURONIDASE
TRANSIENT EXPRESSION
RECOMBINANT PROTEIN PRODUCTION
Transient expression following agroinfiltration of plant tissue was investigated as a system for producing recombinant protein. As a model system, Agrobacterium tumefaciens containing the b-glucuronidase (GUS)gene was vacuum infiltrated into lettuce leaf disks. Infiltration with a suspension of 109 colony forming units/mL followed by incubation for 72 h at 228C in continuous darkness produced a maximum of 0.16
AGROINFILTRATION
LETTUCE
AGROBACTERIUM
BGLUCURONIDASE
TRANSIENT EXPRESSION
RECOMBINANT PROTEIN PRODUCTION