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High-Level Transient Expression of Recombinant Protein in Lettuce

Tipo de material: TextoTextoSeries ; Biotechnology and BioEngineering, 91(7), p.861-671, 2005Trabajos contenidos:
  • Joh, L.D
  • Wroblewski, T
  • Ewing, N.N
  • Van Der Gheyns, J.S
Tema(s): Recursos en línea: Resumen: Transient expression following agroinfiltration of plant tissue was investigated as a system for producing recombinant protein. As a model system, Agrobacterium tumefaciens containing the b-glucuronidase (GUS)gene was vacuum infiltrated into lettuce leaf disks. Infiltration with a suspension of 109 colony forming units/mL followed by incubation for 72 h at 228C in continuous darkness produced a maximum of 0.16
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Transient expression following agroinfiltration of plant tissue was investigated as a system for producing recombinant protein. As a model system, Agrobacterium tumefaciens containing the b-glucuronidase (GUS)gene was vacuum infiltrated into lettuce leaf disks. Infiltration with a suspension of 109 colony forming units/mL followed by incubation for 72 h at 228C in continuous darkness produced a maximum of 0.16

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