Axenic culture of Lemna trisulca L.
Axenic culture of Lemna trisulca L.
- Aquatic Botany, 38(2-3), p.295-301, 1990 .
Methods for sterilizing, subculturing and achieving sustained growth of Lemna trisulca L. in the laboratory are described. Successful growth and a doubling time of 1.9-2.0 days occurred using inorganic carbon at pH 7.8 as the sole carbon source under 400 µmol m-2s-1 cool white fluorescent light at 28±2°C. Cultures were aerated at a rate of 125 ml min-1 with sterile, humidified, room air. The growth medium was unbuffered and replaced once daily at an exponential rate based on the growth of the plant culture.
Methods for sterilizing, subculturing and achieving sustained growth of Lemna trisulca L. in the laboratory are described. Successful growth and a doubling time of 1.9-2.0 days occurred using inorganic carbon at pH 7.8 as the sole carbon source under 400 µmol m-2s-1 cool white fluorescent light at 28±2°C. Cultures were aerated at a rate of 125 ml min-1 with sterile, humidified, room air. The growth medium was unbuffered and replaced once daily at an exponential rate based on the growth of the plant culture.