Axenic culture of Lemna trisulca L. (Record no. 45685)

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fixed length control field 01168nam a2200205Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625140651.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-11469
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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245 10 - TITLE STATEMENT
Title Axenic culture of Lemna trisulca L.
490 0# - SERIES STATEMENT
Volume/sequential designation Aquatic Botany, 38(2-3), p.295-301, 1990
520 3# - SUMMARY, ETC.
Summary, etc. Methods for sterilizing, subculturing and achieving sustained growth of Lemna trisulca L. in the laboratory are described. Successful growth and a doubling time of 1.9-2.0 days occurred using inorganic carbon at pH 7.8 as the sole carbon source under 400 µmol m-2s-1 cool white fluorescent light at 28±2°C. Cultures were aerated at a rate of 125 ml min-1 with sterile, humidified, room air. The growth medium was unbuffered and replaced once daily at an exponential rate based on the growth of the plant culture.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hueberta, David B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mcilraitha, A.L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Shaya, J.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Robinson, G.G.C.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/16serHM7g5QQu0wXcyfzV1mvKF6uh06sJ/view?usp=drivesdk">https://drive.google.com/file/d/16serHM7g5QQu0wXcyfzV1mvKF6uh06sJ/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-11469 25.06.2025 25.06.2025 Documentos solicitados