Influence of scaffold physical properties and stromal cell coculture on hematopoietic differentiation of mouse embryonic stem cells
Influence of scaffold physical properties and stromal cell coculture on hematopoietic differentiation of mouse embryonic stem cells
- BioMaterials, 27, p.6024-6031, 2006 .
Recent studies have suggested that three-dimensional (3D)biomaterial-based scaffolds and dynamic culture conditions could provide significant enhancement in the differentiation efficiency of embryonic stem cells (ESCs). Here we report that scaffold physical properties, like pore size, polymer concentration and compression modulus as well as specific culture conditions, e.g. cell seeding density and coculture with stromal cells can significantly influence hematopoietic differentiation of ESCs. PLLA scaffolds of various polymer concentrations (7.5
EMBRYONIC STEM CELLS
PLGA
SCAFFOLD
HEMATOPOIESIS
Recent studies have suggested that three-dimensional (3D)biomaterial-based scaffolds and dynamic culture conditions could provide significant enhancement in the differentiation efficiency of embryonic stem cells (ESCs). Here we report that scaffold physical properties, like pore size, polymer concentration and compression modulus as well as specific culture conditions, e.g. cell seeding density and coculture with stromal cells can significantly influence hematopoietic differentiation of ESCs. PLLA scaffolds of various polymer concentrations (7.5
EMBRYONIC STEM CELLS
PLGA
SCAFFOLD
HEMATOPOIESIS