Differential screening reveals genes differentially expressed in low- and high-virulence near isogenic Phytophthora sojae lines
Differential screening reveals genes differentially expressed in low- and high-virulence near isogenic Phytophthora sojae lines
- Fungal Genetics and Biology, 43(12), p.826-839, 2006 .
To explore the molecular mechanisms involved in virulence variations in Phytophthora sojae, the low-virulence isolate PS2 was inoculated successively on a resistant soybean (Glycine max)cultivar. After 14 successive inoculations, a high-virulence progeny, termed PS2- vir, was obtained and demonstrated to exhibit lower oospore production. DNA Wngerprinting revealed no large-scale DNA diVerences in PS2 and PS2-vir. A suppression subtractive hybridization (SSH)approach was developed to investigate diVerences in gene expression between PS2 and PS2-vir in the early stages of soybean infection. Of the 323 sequences chosen for examination, 74 utative unigenes were identiWed that exhibit high expression in PS2-vir. These sequences are predicted to encode proteins involved in energy production, protein biosynthesis, cell signaling, cell-wall biogenesis, and transcription regulation. Ten clones were selected for temporal expression analysis using RT-PCR based on the results of the dot-blot screens. The possible genetic mechanisms involved in these phenomena are discussed.
PHYTOPHTHORA SOJAE
TRANSCRIPTION REGULATION
VIRULENCE VARIATION
OOSPORE PRODUCTION
To explore the molecular mechanisms involved in virulence variations in Phytophthora sojae, the low-virulence isolate PS2 was inoculated successively on a resistant soybean (Glycine max)cultivar. After 14 successive inoculations, a high-virulence progeny, termed PS2- vir, was obtained and demonstrated to exhibit lower oospore production. DNA Wngerprinting revealed no large-scale DNA diVerences in PS2 and PS2-vir. A suppression subtractive hybridization (SSH)approach was developed to investigate diVerences in gene expression between PS2 and PS2-vir in the early stages of soybean infection. Of the 323 sequences chosen for examination, 74 utative unigenes were identiWed that exhibit high expression in PS2-vir. These sequences are predicted to encode proteins involved in energy production, protein biosynthesis, cell signaling, cell-wall biogenesis, and transcription regulation. Ten clones were selected for temporal expression analysis using RT-PCR based on the results of the dot-blot screens. The possible genetic mechanisms involved in these phenomena are discussed.
PHYTOPHTHORA SOJAE
TRANSCRIPTION REGULATION
VIRULENCE VARIATION
OOSPORE PRODUCTION