Differential screening reveals genes differentially expressed in low- and high-virulence near isogenic Phytophthora sojae lines (Record no. 47236)

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fixed length control field 02019nam a2200277Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153914.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-13034
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245 10 - TITLE STATEMENT
Title Differential screening reveals genes differentially expressed in low- and high-virulence near isogenic Phytophthora sojae lines
490 0# - SERIES STATEMENT
Volume/sequential designation Fungal Genetics and Biology, 43(12), p.826-839, 2006
520 3# - SUMMARY, ETC.
Summary, etc. To explore the molecular mechanisms involved in virulence variations in Phytophthora sojae, the low-virulence isolate PS2 was inoculated successively on a resistant soybean (Glycine max)cultivar. After 14 successive inoculations, a high-virulence progeny, termed PS2- vir, was obtained and demonstrated to exhibit lower oospore production. DNA Wngerprinting revealed no large-scale DNA diVerences in PS2 and PS2-vir. A suppression subtractive hybridization (SSH)approach was developed to investigate diVerences in gene expression between PS2 and PS2-vir in the early stages of soybean infection. Of the 323 sequences chosen for examination, 74 utative unigenes were identiWed that exhibit high expression in PS2-vir. These sequences are predicted to encode proteins involved in energy production, protein biosynthesis, cell signaling, cell-wall biogenesis, and transcription regulation. Ten clones were selected for temporal expression analysis using RT-PCR based on the results of the dot-blot screens. The possible genetic mechanisms involved in these phenomena are discussed.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PHYTOPHTHORA SOJAE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRANSCRIPTION REGULATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element VIRULENCE VARIATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element OOSPORE PRODUCTION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ziying, W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Yuanchao, W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Xiaoren, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gui, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Zhengguang, Z.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Xiaobo, Z.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1F3wztM52KGN0YW67anGpMrwz-BVKv4gT/view?usp=drivesdk">https://drive.google.com/file/d/1F3wztM52KGN0YW67anGpMrwz-BVKv4gT/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-13034 25.06.2025 25.06.2025 Documentos solicitados