Subtractive hybridization, a technique for extraction of DNA sequences distinguishing two closely related genomes: critical analysis
Subtractive hybridization, a technique for extraction of DNA sequences distinguishing two closely related genomes: critical analysis
- Genetic Analysis: BioMolecular Engineering, 13(2), p.49-58, 1996 .
The present status of genomic DNA subtraction techniques is reviewed. The advantages and disadvantages of the widely-used methods of genome subtrac.tion are discussed. Using the kinetic model of subtractive hybridization developed by us previously (Sverdlov and Ermolaeva, ]993; Sverdlov and Ermolaeva, 1994), the application of genome subtraction to various problems is analyzed. It is concluded that the technique should be further advanced based on subtraction of single-stranded DNAs. This strategy would enable one to efficiently extract target sequences omitting the stage of genome simplification.
SUBTRACTIVE HYBRIDIZATION
ENRICHMENT
KINETICS
The present status of genomic DNA subtraction techniques is reviewed. The advantages and disadvantages of the widely-used methods of genome subtrac.tion are discussed. Using the kinetic model of subtractive hybridization developed by us previously (Sverdlov and Ermolaeva, ]993; Sverdlov and Ermolaeva, 1994), the application of genome subtraction to various problems is analyzed. It is concluded that the technique should be further advanced based on subtraction of single-stranded DNAs. This strategy would enable one to efficiently extract target sequences omitting the stage of genome simplification.
SUBTRACTIVE HYBRIDIZATION
ENRICHMENT
KINETICS