Subtractive hybridization, a technique for extraction of DNA sequences distinguishing two closely related genomes: critical analysis (Record no. 48331)

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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-14136
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245 10 - TITLE STATEMENT
Title Subtractive hybridization, a technique for extraction of DNA sequences distinguishing two closely related genomes: critical analysis
490 0# - SERIES STATEMENT
Volume/sequential designation Genetic Analysis: BioMolecular Engineering, 13(2), p.49-58, 1996
520 3# - SUMMARY, ETC.
Summary, etc. The present status of genomic DNA subtraction techniques is reviewed. The advantages and disadvantages of the widely-used methods of genome subtrac.tion are discussed. Using the kinetic model of subtractive hybridization developed by us previously (Sverdlov and Ermolaeva, ]993; Sverdlov and Ermolaeva, 1994), the application of genome subtraction to various problems is analyzed. It is concluded that the technique should be further advanced based on subtraction of single-stranded DNAs. This strategy would enable one to efficiently extract target sequences omitting the stage of genome simplification.
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Topical term or geographic name entry element SUBTRACTIVE HYBRIDIZATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ENRICHMENT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element KINETICS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ermolaeva, O.D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sverdlov, E.D.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1BDeec3jdMNSyZ5OXU_tTk-O1zdhvT6fD/view?usp=drivesdk">https://drive.google.com/file/d/1BDeec3jdMNSyZ5OXU_tTk-O1zdhvT6fD/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-14136 25.06.2025 25.06.2025 Documentos solicitados