Tandem Affinity Purification and Mass Spectrometry (TAP-MS)for the Analysis of Protein Complexes
Tandem Affinity Purification and Mass Spectrometry (TAP-MS)for the Analysis of Protein Complexes
- Current Protocols in protein Science, 96, p. https://doi.org/10.1002/cpps.84, 2019 .
Affinity purification followed by mass spectrometry has become the technique of choice to identify binding partners in biochemical complexes isolated from a physiologic cellular context. In this report we detail our protocol for tandem affinity purification (TAP)primarily based on the use of the FLAG and HA peptide epitopes, with a particular emphasis on factors affecting yield and specificity, as well as steps to implement an automated version of the TAP procedure.
Affinity purification followed by mass spectrometry has become the technique of choice to identify binding partners in biochemical complexes isolated from a physiologic cellular context. In this report we detail our protocol for tandem affinity purification (TAP)primarily based on the use of the FLAG and HA peptide epitopes, with a particular emphasis on factors affecting yield and specificity, as well as steps to implement an automated version of the TAP procedure.