Tandem Affinity Purification and Mass Spectrometry (TAP-MS)for the Analysis of Protein Complexes (Record no. 51967)

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control field 20250625160217.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-17806
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Title Tandem Affinity Purification and Mass Spectrometry (TAP-MS)for the Analysis of Protein Complexes
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Volume/sequential designation Current Protocols in protein Science, 96, p. https://doi.org/10.1002/cpps.84, 2019
520 3# - SUMMARY, ETC.
Summary, etc. Affinity purification followed by mass spectrometry has become the technique of choice to identify binding partners in biochemical complexes isolated from a physiologic cellular context. In this report we detail our protocol for tandem affinity purification (TAP)primarily based on the use of the FLAG and HA peptide epitopes, with a particular emphasis on factors affecting yield and specificity, as well as steps to implement an automated version of the TAP procedure.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Adelmant, G.
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Personal name Garg, B. K.
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Personal name Tavares, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Card, J. D.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Marto, J. A.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1LgnnvgFzFv1Kpm0LFB9kXTYFjoc6X8Ot/view?usp=drivesdk">https://drive.google.com/file/d/1LgnnvgFzFv1Kpm0LFB9kXTYFjoc6X8Ot/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-17806 25.06.2025 25.06.2025 Documentos solicitados