Specific selection of embryogenic cells lines in Agrostis alba L. using the proline analog thioproline
Specific selection of embryogenic cells lines in Agrostis alba L. using the proline analog thioproline
- Plant Science, 79(2), p.259-263, 1991 .
The proline analog, thioproline was used to specifically isolate cell lines of Agrostis alba L. with the capacity to initiate embryogenic callus from mature seed. During the selection in the presence of 2,4-dichlorophenoxy acetic acid (2,4-D)as the auxin in standard Murashige and Skoog (MS)medium, only cell lines forming highly embryogenic callus proliferated. On the other hand, growth of cell lines forming non-embryogenic callus were inhibited. A thioproline concentration of 1.0 mM was particularly effective in specifically selecting embryogenic callus cell lines which were not inhibited in growth. At the same time 1.0 mM thioproline effectively inhibited the growth of non-embryogenic callus cell lines. The possible physiological mechanism by which the embryogenic callus cell lines were tolerant to thioproline is discussed.
AGROSTIS ALBA L.
EMBRYOGENIC CALLUS
THIOPROLINE
PROLINE
The proline analog, thioproline was used to specifically isolate cell lines of Agrostis alba L. with the capacity to initiate embryogenic callus from mature seed. During the selection in the presence of 2,4-dichlorophenoxy acetic acid (2,4-D)as the auxin in standard Murashige and Skoog (MS)medium, only cell lines forming highly embryogenic callus proliferated. On the other hand, growth of cell lines forming non-embryogenic callus were inhibited. A thioproline concentration of 1.0 mM was particularly effective in specifically selecting embryogenic callus cell lines which were not inhibited in growth. At the same time 1.0 mM thioproline effectively inhibited the growth of non-embryogenic callus cell lines. The possible physiological mechanism by which the embryogenic callus cell lines were tolerant to thioproline is discussed.
AGROSTIS ALBA L.
EMBRYOGENIC CALLUS
THIOPROLINE
PROLINE