Specific selection of embryogenic cells lines in Agrostis alba L. using the proline analog thioproline (Record no. 54649)

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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20562
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245 10 - TITLE STATEMENT
Title Specific selection of embryogenic cells lines in Agrostis alba L. using the proline analog thioproline
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Science, 79(2), p.259-263, 1991
520 3# - SUMMARY, ETC.
Summary, etc. The proline analog, thioproline was used to specifically isolate cell lines of Agrostis alba L. with the capacity to initiate embryogenic callus from mature seed. During the selection in the presence of 2,4-dichlorophenoxy acetic acid (2,4-D)as the auxin in standard Murashige and Skoog (MS)medium, only cell lines forming highly embryogenic callus proliferated. On the other hand, growth of cell lines forming non-embryogenic callus were inhibited. A thioproline concentration of 1.0 mM was particularly effective in specifically selecting embryogenic callus cell lines which were not inhibited in growth. At the same time 1.0 mM thioproline effectively inhibited the growth of non-embryogenic callus cell lines. The possible physiological mechanism by which the embryogenic callus cell lines were tolerant to thioproline is discussed.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AGROSTIS ALBA L.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element EMBRYOGENIC CALLUS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element THIOPROLINE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PROLINE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Shetty, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Asano, Y.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1UerBcJVhBSB7mhetOeTg6QA4JW6Y48L3/view?usp=drivesdk">https://drive.google.com/file/d/1UerBcJVhBSB7mhetOeTg6QA4JW6Y48L3/view?usp=drivesdk</a>
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