Chloroplast expression of His-tagged GUS-fusions: a general strategy to overproduce and purify foreign proteins using transplastomic plants as bioreactors (Record no. 45090)

MARC details
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fixed length control field 01377nam a2200241Ia 4500
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control field MX-MdCICY
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control field 20250625140639.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-10859
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Title Chloroplast expression of His-tagged GUS-fusions: a general strategy to overproduce and purify foreign proteins using transplastomic plants as bioreactors
490 0# - SERIES STATEMENT
Volume/sequential designation Molecular Breeding, 11, p.49-58, 2003
520 3# - SUMMARY, ETC.
Summary, etc. High level expression and efficient recovery of recombinant protein are two main critical factors that determine the use of transgenic plants as natural bioreactors to produce foreign proteins for industrial applications. We demonstrate here the potential of a new strategy involving chloroplast transformation, GUS-fusions and affinitytag based chromatography to overexpress and purify a human therapeutic protein, interferon gamma (IFN-g)in tobacco plants. Our results show that IFN-g accumulation reaches up to 6
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AFFINITY CHROMATOGRAPHY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHLOROPLAST EXPRESSION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GUS-FUSION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HIS-TAG
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element INTERFERON GAMMA
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Leelavathi, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Reddy, V.S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1Jceaelly_0w7PCDzOuV4-brym8gC7e2_/view?usp=drivesdk">https://drive.google.com/file/d/1Jceaelly_0w7PCDzOuV4-brym8gC7e2_/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-10859 25.06.2025 25.06.2025 Documentos solicitados