Image from Google Jackets

Chloroplast expression of His-tagged GUS-fusions: a general strategy to overproduce and purify foreign proteins using transplastomic plants as bioreactors

Tipo de material: TextoTextoSeries ; Molecular Breeding, 11, p.49-58, 2003Trabajos contenidos:
  • Leelavathi, S
  • Reddy, V.S
Tema(s): Recursos en línea: Resumen: High level expression and efficient recovery of recombinant protein are two main critical factors that determine the use of transgenic plants as natural bioreactors to produce foreign proteins for industrial applications. We demonstrate here the potential of a new strategy involving chloroplast transformation, GUS-fusions and affinitytag based chromatography to overexpress and purify a human therapeutic protein, interferon gamma (IFN-g)in tobacco plants. Our results show that IFN-g accumulation reaches up to 6
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Collection Call number Status Date due Barcode
Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-10859 (Browse shelf(Opens below)) Available

High level expression and efficient recovery of recombinant protein are two main critical factors that determine the use of transgenic plants as natural bioreactors to produce foreign proteins for industrial applications. We demonstrate here the potential of a new strategy involving chloroplast transformation, GUS-fusions and affinitytag based chromatography to overexpress and purify a human therapeutic protein, interferon gamma (IFN-g)in tobacco plants. Our results show that IFN-g accumulation reaches up to 6

There are no comments on this title.

to post a comment.