Cryopreservation and metabolic profiling analysis of Arabidopsis T87 suspension-cultured cells (Record no. 47400)

MARC details
000 -LEADER
fixed length control field 01498nam a2200301Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153918.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-13199
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250602s9999 xx |||||s2 |||| ||und|d
245 10 - TITLE STATEMENT
Title Cryopreservation and metabolic profiling analysis of Arabidopsis T87 suspension-cultured cells
490 0# - SERIES STATEMENT
Volume/sequential designation CryoLetters, 29(5), p.427-436, 2008
520 3# - SUMMARY, ETC.
Summary, etc. We established a simple cryopreservation protocol for Arabidopsis T87 cells using an encapsulation-dehydration method. T87 cells were encapsulated into alginate beads containing 2 M glycerol and 0.4 M sucrose. Alginate beads containing T87 cells were dehydrated with silica gel for 2 h (to c. 0.7 g H2O g DW-1)followed by immersed in LN. After rewarming at 35°C for 3 min and 1-d incubation under continuous illumination at 22°C, cryopreserved T87 cells exhibited considerable regrowth. Exponentially-grown 7-d-old T87 cells regrew more vigorously (86
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ARABIDOPSIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CRYOPRESERVATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ENCAPSULATION-DEHYDRATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SUSPENSION-CULTURED CELLS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element METABOLIC PROFILING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GC-TOF-MS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ogawa, Y.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Suzuki, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sakurai, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Aoki, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Saito, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Shibata, D.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1vnN9yApo8K5_0csja9RQLU2Y6R1rH72I/view?usp=drivesdk">https://drive.google.com/file/d/1vnN9yApo8K5_0csja9RQLU2Y6R1rH72I/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-13199 25.06.2025 25.06.2025 Documentos solicitados