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Cryopreservation and metabolic profiling analysis of Arabidopsis T87 suspension-cultured cells

Tipo de material: TextoTextoSeries ; CryoLetters, 29(5), p.427-436, 2008Trabajos contenidos:
  • Ogawa, Y
  • Suzuki, H
  • Sakurai, N
  • Aoki, K
  • Saito, K
  • Shibata, D
Tema(s): Recursos en línea: Resumen: We established a simple cryopreservation protocol for Arabidopsis T87 cells using an encapsulation-dehydration method. T87 cells were encapsulated into alginate beads containing 2 M glycerol and 0.4 M sucrose. Alginate beads containing T87 cells were dehydrated with silica gel for 2 h (to c. 0.7 g H2O g DW-1)followed by immersed in LN. After rewarming at 35°C for 3 min and 1-d incubation under continuous illumination at 22°C, cryopreserved T87 cells exhibited considerable regrowth. Exponentially-grown 7-d-old T87 cells regrew more vigorously (86
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We established a simple cryopreservation protocol for Arabidopsis T87 cells using an encapsulation-dehydration method. T87 cells were encapsulated into alginate beads containing 2 M glycerol and 0.4 M sucrose. Alginate beads containing T87 cells were dehydrated with silica gel for 2 h (to c. 0.7 g H2O g DW-1)followed by immersed in LN. After rewarming at 35°C for 3 min and 1-d incubation under continuous illumination at 22°C, cryopreserved T87 cells exhibited considerable regrowth. Exponentially-grown 7-d-old T87 cells regrew more vigorously (86

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