MARC details
| 000 -LEADER |
|---|
| fixed length control field |
01699nam a2200337Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20250625153945.0 |
| 040 ## - CATALOGING SOURCE |
|---|
| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-14660 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Three Methods for the Introduction of Foreign DNA intoAgrobacterium |
| 490 0# - SERIES STATEMENT |
|---|
| Volume/sequential designation |
Methods in Molecular Biology, 343, p.43-53, 2009 |
| 520 3# - SUMMARY, ETC. |
|---|
| Summary, etc. |
The genetic manipulation of Agrobacterium tumefaciensis used to facilitate studies of bacterial gene functions or as a first step in introducing genetic material into transformable plant cells through the use of T-DNA binary vectors. Three methods are commonly used. Transformation with purified plasmid can be done with either electroporation or a simple freeze/thaw transformation method. Alternatively, a mobilizable plasmid can be placed into Agrobacteriumusing the triparental mating method. Here we present three detailed protocols for Agrobacteriumstrain construction using electroporation, the freeze/thaw method of transformation, and triparental mating. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
ELECTROPORATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
FREEZE/THAW |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
TRIPARENTAL MATING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
CONJUGATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
INCOM-PATIBILITY GROUP |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
SELECTIVE PLATES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
COUNTERSELECTION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
DONOR |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
RECIPIENT |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
TRANSCONJUGANT |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
SELF-TRANSMISSIBLE PLASMID |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
ORIGIN OF TRANSFER (ORIT). |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Wise, A.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Liu, Z. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Binns, A.N. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/19roaQ8d2vcliIUkWpFdlISlqf3bhTh0A/view?usp=drivesdk">https://drive.google.com/file/d/19roaQ8d2vcliIUkWpFdlISlqf3bhTh0A/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
