Three Methods for the Introduction of Foreign DNA intoAgrobacterium

Tipo de material: Texto

TextoSeries ; Methods in Molecular Biology, 343, p.43-53, 2009Trabajos contenidos:

Wise, A.A
Liu, Z
Binns, A.N

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Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx

Resumen: The genetic manipulation of Agrobacterium tumefaciensis used to facilitate studies of bacterial gene functions or as a first step in introducing genetic material into transformable plant cells through the use of T-DNA binary vectors. Three methods are commonly used. Transformation with purified plasmid can be done with either electroporation or a simple freeze/thaw transformation method. Alternatively, a mobilizable plasmid can be placed into Agrobacteriumusing the triparental mating method. Here we present three detailed protocols for Agrobacteriumstrain construction using electroporation, the freeze/thaw method of transformation, and triparental mating.

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The genetic manipulation of Agrobacterium tumefaciensis used to facilitate studies of bacterial gene functions or as a first step in introducing genetic material into transformable plant cells through the use of T-DNA binary vectors. Three methods are commonly used. Transformation with purified plasmid can be done with either electroporation or a simple freeze/thaw transformation method. Alternatively, a mobilizable plasmid can be placed into Agrobacteriumusing the triparental mating method. Here we present three detailed protocols for Agrobacteriumstrain construction using electroporation, the freeze/thaw method of transformation, and triparental mating.

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