Analysis of Protein Changes Using Two-Dimensional Difference Gel Electrophoresis (Record no. 49220)

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fixed length control field 01516nam a2200217Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
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control field 20250625153953.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-15038
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245 10 - TITLE STATEMENT
Title Analysis of Protein Changes Using Two-Dimensional Difference Gel Electrophoresis
490 0# - SERIES STATEMENT
Volume/sequential designation Method Mol. Biol. Mol. Tox. Protocols, 1105, p.17-30, 2014
520 3# - SUMMARY, ETC.
Summary, etc. A protocol for protein analysis using two-dimensional difference gel electrophoresis (2D-DIGE)is described. 2D-DIGE is one of the most popular and versatile methods of protein separation among rapidly increasing proteomics technologies. Similar to traditional two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the proteins are separated based on their charges and molecular weight by 2D-DIGE. Different from 2D-PAGE, proteins are pre-labeled with different fl uorescent and different protein samples are run in one gel by this method. Therefore, 2D-DIGE not only carries the advantages of 2D-PAGE but also eliminates gel-to-gel variation and achieves high resolution, sensitivity, and reproducibility.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TWO-DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PROTEIN SEPARATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PROTEOMICS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gao, W.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1U6IQUr5SeaUBbagwiD4bqFQJsSFBQ_ho/view?usp=drivesdk">https://drive.google.com/file/d/1U6IQUr5SeaUBbagwiD4bqFQJsSFBQ_ho/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-15038 25.06.2025 25.06.2025 Documentos solicitados