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Analysis of Protein Changes Using Two-Dimensional Difference Gel Electrophoresis

Tipo de material: TextoTextoSeries ; Method Mol. Biol. Mol. Tox. Protocols, 1105, p.17-30, 2014Trabajos contenidos:
  • Gao, W
Tema(s): Recursos en línea: Resumen: A protocol for protein analysis using two-dimensional difference gel electrophoresis (2D-DIGE)is described. 2D-DIGE is one of the most popular and versatile methods of protein separation among rapidly increasing proteomics technologies. Similar to traditional two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the proteins are separated based on their charges and molecular weight by 2D-DIGE. Different from 2D-PAGE, proteins are pre-labeled with different fl uorescent and different protein samples are run in one gel by this method. Therefore, 2D-DIGE not only carries the advantages of 2D-PAGE but also eliminates gel-to-gel variation and achieves high resolution, sensitivity, and reproducibility.
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A protocol for protein analysis using two-dimensional difference gel electrophoresis (2D-DIGE)is described. 2D-DIGE is one of the most popular and versatile methods of protein separation among rapidly increasing proteomics technologies. Similar to traditional two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the proteins are separated based on their charges and molecular weight by 2D-DIGE. Different from 2D-PAGE, proteins are pre-labeled with different fl uorescent and different protein samples are run in one gel by this method. Therefore, 2D-DIGE not only carries the advantages of 2D-PAGE but also eliminates gel-to-gel variation and achieves high resolution, sensitivity, and reproducibility.

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