Silver staining of DNA in polyacrylamide gels: Linearity and effect of fragment size (Record no. 50290)

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fixed length control field 01270nam a2200193Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160145.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16115
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245 10 - TITLE STATEMENT
Title Silver staining of DNA in polyacrylamide gels: Linearity and effect of fragment size
490 0# - SERIES STATEMENT
Volume/sequential designation Electrophoresis, 3(1), p.24-26, 1982
520 3# - SUMMARY, ETC.
Summary, etc. A simplified, inexpensive silver stain for proteins in polyacrylamide gels also stains DNA with a sensitivity of 1 ng (< 0.03 ng/mm2). Sensitivity of detection is superior to ethidium bromide and, for 11 double-stranded fragments of OX 174 DNA, staining is linear with respect to concentration. Surprisingly, the slope of staining is similar for pieces of DNA larger than 300 base pairs, but is greater for fragments of smaller size. Staining of DNA with silver is a practical alternative to visualization with ethidium bromide and may serve as a tool or elucidating the basis of silver staining.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element POLYACRYLAMIDE GEL ELECTROPHORESIS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Goldman, D
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Merril, Cr
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1rxmoz4rpAAdv6-Nj07wZAJREtEqJhHmU/view?usp=drivesdk">https://drive.google.com/file/d/1rxmoz4rpAAdv6-Nj07wZAJREtEqJhHmU/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16115 25.06.2025 25.06.2025 Documentos solicitados