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Silver staining of DNA in polyacrylamide gels: Linearity and effect of fragment size

Tipo de material: TextoTextoSeries ; Electrophoresis, 3(1), p.24-26, 1982Trabajos contenidos:
  • Goldman, D
  • Merril, Cr
Tema(s): Recursos en línea: Resumen: A simplified, inexpensive silver stain for proteins in polyacrylamide gels also stains DNA with a sensitivity of 1 ng (< 0.03 ng/mm2). Sensitivity of detection is superior to ethidium bromide and, for 11 double-stranded fragments of OX 174 DNA, staining is linear with respect to concentration. Surprisingly, the slope of staining is similar for pieces of DNA larger than 300 base pairs, but is greater for fragments of smaller size. Staining of DNA with silver is a practical alternative to visualization with ethidium bromide and may serve as a tool or elucidating the basis of silver staining.
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Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-16115 (Browse shelf(Opens below)) Available

A simplified, inexpensive silver stain for proteins in polyacrylamide gels also stains DNA with a sensitivity of 1 ng (< 0.03 ng/mm2). Sensitivity of detection is superior to ethidium bromide and, for 11 double-stranded fragments of OX 174 DNA, staining is linear with respect to concentration. Surprisingly, the slope of staining is similar for pieces of DNA larger than 300 base pairs, but is greater for fragments of smaller size. Staining of DNA with silver is a practical alternative to visualization with ethidium bromide and may serve as a tool or elucidating the basis of silver staining.

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