Cytochrome P-450-catalysed monoterpenoid oxidation in catmint (Nepeta racemosa)and avocado (Persea americana); evidence for related enzymes with different activities (Record no. 51322)

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control field MX-MdCICY
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control field 20250625160205.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-17159
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Title Cytochrome P-450-catalysed monoterpenoid oxidation in catmint (Nepeta racemosa)and avocado (Persea americana); evidence for related enzymes with different activities
490 0# - SERIES STATEMENT
Volume/sequential designation Biochimica et Biophysica Acta, 1201(1), p.94-100, 1994
520 3# - SUMMARY, ETC.
Summary, etc. A cytochrome P-450 present in ripening avocado (Persea americana)fruit mesocarp (CYTP71A1)had previously been shown to metabolize the monoterpenoids nerol and geraniol (Hallahan et al. (1992)Plant Physiol. 98, 1290-1297). Using DNA encoding CYP71A1 as a hybridization probe, we have shown by Southern analysis that a related gene is present in the catmint, Nepeta racemosa. RNA blot analysis, together with Western analysis of catmint leaf polypeptides using avocado cyt P-450 antiserum, showed that a closely related gene is expressed in catmint leaves. Cytochrome P-450 in catmint microsomes catalysed the specific hydroxylation of nerol and geraniol at C-10, whereas avocado CYP71A1, in either avocado microsomes or heterologously expressed in yeast, catalysed 2,3- or 6,7-epoxidation of these substrates. These results suggest that orthologous genes of the CYP71 family are expressed in these two plant species, but catalyse dissimilar reactions with monoterpenoid substrates.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CYTOCHROME P-450
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PLANT ENZYME
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SUBSTRATE SPECIFICITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MONOTERPENOID
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GENES
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Topical term or geographic name entry element HYBRIDIZATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element (N. RACEMOSA)
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element (P. AMERICANA)
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hallahan, D. L.
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Personal name Lau, S. M. C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Harder, P. A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Smiley, D. W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Dawson, G. W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Pickett, J. A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name O'Keefe, D. P.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1A3M-A9XFSMREcTjDp2E0adgqHVjxQHRO/view?usp=drivesdk">https://drive.google.com/file/d/1A3M-A9XFSMREcTjDp2E0adgqHVjxQHRO/view?usp=drivesdk</a>
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