Cytochrome P-450-catalysed monoterpenoid oxidation in catmint (Nepeta racemosa)and avocado (Persea americana); evidence for related enzymes with different activities
Tipo de material:
TextoSeries ; Biochimica et Biophysica Acta, 1201(1), p.94-100, 1994Trabajos contenidos: - Hallahan, D. L
- Lau, S. M. C
- Harder, P. A
- Smiley, D. W
- Dawson, G. W
- Pickett, J. A
- O'Keefe, D. P
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A cytochrome P-450 present in ripening avocado (Persea americana)fruit mesocarp (CYTP71A1)had previously been shown to metabolize the monoterpenoids nerol and geraniol (Hallahan et al. (1992)Plant Physiol. 98, 1290-1297). Using DNA encoding CYP71A1 as a hybridization probe, we have shown by Southern analysis that a related gene is present in the catmint, Nepeta racemosa. RNA blot analysis, together with Western analysis of catmint leaf polypeptides using avocado cyt P-450 antiserum, showed that a closely related gene is expressed in catmint leaves. Cytochrome P-450 in catmint microsomes catalysed the specific hydroxylation of nerol and geraniol at C-10, whereas avocado CYP71A1, in either avocado microsomes or heterologously expressed in yeast, catalysed 2,3- or 6,7-epoxidation of these substrates. These results suggest that orthologous genes of the CYP71 family are expressed in these two plant species, but catalyse dissimilar reactions with monoterpenoid substrates.
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