Directed evolution of Thermomyces lanuginosus lipase to enhance methanol tolerance for efficient production of biodiesel from waste grease (Record no. 54098)
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| fixed length control field | 01940nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625162440.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-19993 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Directed evolution of Thermomyces lanuginosus lipase to enhance methanol tolerance for efficient production of biodiesel from waste grease |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Bioresource Technology, 245, p.1491-1497, 2017 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Engineering a methanol tolerant lipase is of great importance in biodiesel production. Here, the first semi-rational method for directed enzyme evolution to enhance methanol tolerance by targeting high B-factor residues for iterative saturation mutagenesis (ISM)is reported. The best double mutant, TLL-S105C/D27R, retained 71 percent of its original activity after incubation in methanol, showing 30 percent greater methanol tolerance than TLL. TLL-S105C/D27R also displayed 27 percent higher activity over TLL. Structure modelling suggested that the increased stability of TLL-S105C/D27R was caused by the formation of a new hydrogen bond which stabilized the protein structure. E. coli (TLL-S105C/D27R)-catalyzed biotransformation of waste grease produced biodiesel in 81 percent yield in 8h, showing improvement over the 67 percent yield for E. coli (TLL), while retaining 92 percent productivity after 4 cycles of biotransformation of waste grease. The engineered TLL mutant shows high potential for commercial biodiesel production |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | B-FACTOR |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | BIODIESEL |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | DIRECTED EVOLUTION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | METHANOL TOLERANCE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | THERMOMYCES LANUGINOSUS LIPASE |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tian, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tai, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chua, B. J. W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Li, Z. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1jRZGswgxUZNQE8rlVySk5R5BFVuFyWPW/view?usp=drivesdk">https://drive.google.com/file/d/1jRZGswgxUZNQE8rlVySk5R5BFVuFyWPW/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-19993 | 25.06.2025 | 25.06.2025 | Documentos solicitados |