Preparation of carotenoid cleavage dioxygenases for X-ray crystallography (Record no. 54366)

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control field MX-MdCICY
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control field 20250625162445.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-20267
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Title Preparation of carotenoid cleavage dioxygenases for X-ray crystallography
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Volume/sequential designation Methods in Enzymology, 671, p.243-271, 2022
520 3# - SUMMARY, ETC.
Summary, etc. Carotenoid cleavage dioxygenases (CCDs)constitute a superfamily of enzymes that are found in all domains of life where they play key roles in the metabolism of carotenoids and apocarotenoids as well as certain phenylpropanoids such as resveratrol. Interest in these enzymes stems not only from their biological importance but also from their remarkable catalytic properties including their regioselectivity, their ability to accommodate diverse substrates, and the additional activities (e.g., isomerase)that some of these enzyme possess. X-ray crystallography is a key experimental approach that has allowed detailed investigation into the structural basis behind the interesting biochemical features of these enzymes. Here, we describe approaches used by our lab that have proven successful in generating single crystals of these enzymes in resting or ligand-bound states for high-resolution X-ray diffraction analysis.
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Topical term or geographic name entry element APOCAROTENOID
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Topical term or geographic name entry element CAROTENOID
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Topical term or geographic name entry element CLEAVAGE DIOXYGENASE
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Topical term or geographic name entry element COBALT
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Topical term or geographic name entry element CRYSTALLIZATION
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Topical term or geographic name entry element ENZYME
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Topical term or geographic name entry element METALLOENZYME
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Topical term or geographic name entry element NON-HEME IRON
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Topical term or geographic name entry element X-RAY CRYSTALLOGRAPHY
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Personal name Daruwalla, A.
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Personal name Sui, X.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kiser, P. D.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1DQnWn-9WAVoyFtGX57OQuiZoh-8fIQZM/view?usp=drivesdk">https://drive.google.com/file/d/1DQnWn-9WAVoyFtGX57OQuiZoh-8fIQZM/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-20267 25.06.2025 25.06.2025 Documentos solicitados