Comparison of yeast cell protein solubilization procedures for two-dimensional electrophoresis

Tipo de material: Texto

TextoSeries ; Electrophoresis, 20, p.826-829, 1999Trabajos contenidos:

Harder, A
Wildgruber, R
Nawrocki, A
Fey, S.J
Larsen, P.M

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Resumen: Three different procedures for the solubilization of yeast (S. cerevisiae)cell proteins were compared on the basis of the obtained two-dimensional (2-D)polypeptide patterns. Major emphasis was laid on minimizing handling steps, protein modification or degradation, and quantitative loss of high molecular mass proteins. The procedures employed were sonication, followed by (i)protein solubilization with "standard" lysis buffer (9 M urea, 2

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Three different procedures for the solubilization of yeast (S. cerevisiae)cell proteins were compared on the basis of the obtained two-dimensional (2-D)polypeptide patterns. Major emphasis was laid on minimizing handling steps, protein modification or degradation, and quantitative loss of high molecular mass proteins. The procedures employed were sonication, followed by (i)protein solubilization with "standard" lysis buffer (9 M urea, 2

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