REMI-induced mutants ofMycosphaerella zeae-maydislacking the polyketide PM-toxin are deficient in pathogenesis to corn

Tipo de material: Texto

TextoSeries ; Physiological and Molecular Plant Pathology, 52(1), p.53-66, 1998Trabajos contenidos:

Yun, S.-H
Turgeon, B.G
Yoder, O.C

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Resumen: The polyketide PM-toxin, produced byMycosphaerella zeae-maydis, is specifically active against corn containing Texas male sterile (T)cytoplasm, as is the fungus itself. To determine if PM-toxin is required for disease development,Tox-mutants were generated using the restriction enzyme-mediated integration (REMI)strategy, which is designed to tag the mutations it creates. As a mutagenic procedure, REMI was highly efficient, yielding five stable PM-toxin-deficient mutants among 504 transformants recovered. AllTox-mutants lost the ability to cause disease on T-cytoplasm corn, establishing a role for PM-toxin in pathogenesis. Examination of genomic DNAs from theTox-mutants revealed one that carried the transformation vector at multiple insertion sites and two that had no apparent ORFs at single vector insertion sites; three sustained deletions.

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The polyketide PM-toxin, produced byMycosphaerella zeae-maydis, is specifically active against corn containing Texas male sterile (T)cytoplasm, as is the fungus itself. To determine if PM-toxin is required for disease development,Tox-mutants were generated using the restriction enzyme-mediated integration (REMI)strategy, which is designed to tag the mutations it creates. As a mutagenic procedure, REMI was highly efficient, yielding five stable PM-toxin-deficient mutants among 504 transformants recovered. AllTox-mutants lost the ability to cause disease on T-cytoplasm corn, establishing a role for PM-toxin in pathogenesis. Examination of genomic DNAs from theTox-mutants revealed one that carried the transformation vector at multiple insertion sites and two that had no apparent ORFs at single vector insertion sites; three sustained deletions.

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