Isolation of RNA and DNA fragments using diatomaceous earth1

Tipo de material: Texto

TextoSeries ; Biotechnology Techniques, 12(7), p.549-552, 1998Trabajos contenidos:

Koo, K
Foegeding, P.M
Swaisgood, H.E

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Resumen: A series of simple and phenol-free silica-based protocols for isolating and cleaning RNA or DNA fragments from different sources were developed. Cytoplasmic RNA isolated from hybridoma cells by this method was used in RTPCR. DNA fragments obtained using this protocol were suitable for further subcloning, gene transformation and DNA sequencing.

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A series of simple and phenol-free silica-based protocols for isolating and cleaning RNA or DNA fragments from different sources were developed. Cytoplasmic RNA isolated from hybridoma cells by this method was used in RTPCR. DNA fragments obtained using this protocol were suitable for further subcloning, gene transformation and DNA sequencing.

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