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Apolar chromatography on Sephadex LH-20 combined with high-speed counter-current chromatography High yield strategy for structurally closely related analytes-Destruxin derivatives from Metarhizium anisopliae as a case study

Tipo de material: TextoTextoSeries ; Journal of Chromatography A, 1117(1), p.67-73, 2006Trabajos contenidos:
  • Seger, C
  • Eberhart, K
  • Sturm, S
  • Strasser, H
  • Stuppner, H
Tema(s): Recursos en línea: Resumen: A novel high yield isolation procedure for lipophilic cyclic peptide derivatives is presented. Destruxin (dtx)A, B, D, E, and E-diol retrieval from Metarhizium anisopliae culture broth was achieved with a three-step purification protocol. After liquid-liquid extraction column chromatography over Sephadex LH-20 served as enrichment step. High-speed counter-current chromatography (HSCCC)was used for the final purification.Within the first chromatographic step dtx D and dtx E-diol were separated in purities exceeding 90
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A novel high yield isolation procedure for lipophilic cyclic peptide derivatives is presented. Destruxin (dtx)A, B, D, E, and E-diol retrieval from Metarhizium anisopliae culture broth was achieved with a three-step purification protocol. After liquid-liquid extraction column chromatography over Sephadex LH-20 served as enrichment step. High-speed counter-current chromatography (HSCCC)was used for the final purification.Within the first chromatographic step dtx D and dtx E-diol were separated in purities exceeding 90

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