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Starch Metabolism in Shoot-forming Tobacco Callus

Tipo de material: TextoTextoSeries ; Journal of Experimental Botany, 25(85), p.288-294, 1974Trabajos contenidos:
  • Thorpe, T.A
  • Meier, D.D
Recursos en línea: Resumen: Starch, free sugars and protein contents, and the specific activities of enzymes of starch metabolism were determined in tobacco callus cultured under shoot-forming and non-shootforming conditions. Shoot-forming cultures contained higher levels of starch, free sugars and protein. Shoot-forming cultures had higher specific activities for starch-synthesising enzymes throughout culture. On the other hand, higher levels of activity for starch-degrading enzymes in shoot-forming tissues were only observed during organized development. The role of phosphorylase in the cultured tissue was not clear.
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Starch, free sugars and protein contents, and the specific activities of enzymes of starch metabolism were determined in tobacco callus cultured under shoot-forming and non-shootforming conditions. Shoot-forming cultures contained higher levels of starch, free sugars and protein. Shoot-forming cultures had higher specific activities for starch-synthesising enzymes throughout culture. On the other hand, higher levels of activity for starch-degrading enzymes in shoot-forming tissues were only observed during organized development. The role of phosphorylase in the cultured tissue was not clear.

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