Studies on preparation and viability of Phytophthora parasitica spheroplasts

Tipo de material: Texto

TextoSeries ; Trans. Br. Mycol. Soc, 89(2), p.213-220, 1987Trabajos contenidos:

Jahnke, K.D
Leipoldt, G
Prell, H.H

Recursos en línea:

Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx

Resumen: Spheroplasts were obtained from Phytophthora parasitica mycelium by digestion with Novozym 234 using 0'35 M CaCI 2 (pH 5'7)as initial osmotic stabilizer. Spheroplast yield decreased rapidly with increasing age of the mycelium. Young mycelium gave highest yields of9'3 ±4'5 x 106 cells per Petri dish. Regeneration was investigated on synthetic and complex media in the presence of different osmotic stabilizers. Survival on solid and in liquid media was enhanced in the presence of CaCI2 in addition to mannitol as osmotic stabilizer. When spheroplasts were spread on agar containing 0'1 M CaCI 2 plus 0"4 M mannitol as osmotica the regeneration rate was 12 ± 4 x cent. After embedding in soft agarose stabilized with o-8 M mannitol spheroplast regeneration was 19 ± 6 x pent. Incubation in osmotically stabilized liquid medium resulted in a rapid loss of viability. High concentrations of CaCI 2 as osmotic stabilizer caused abnormal growth of spheroplasts which eventually gave rise to normal mycelium.

Tags from this library: No tags from this library for this title. Log in to add tags.

Average rating: 0.0 (0 votes)

Holdings
Item type	Current library	Collection	Call number	Status	Date due	Barcode
Documentos solicitados	CICY Documento préstamo interbibliotecario	Ref1	B-11959 (Browse shelf(Opens below))	Available

Browsing CICY shelves, Shelving location: Documento préstamo interbibliotecario, Collection: Ref1 Close shelf browser (Hides shelf browser)

Previous	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	Next
Previous	B-11956 Anti-HIV activity of medicinal plant extracts	B-11957 The role of phosphorylatable serine residues in the DNA-binding domain of Arabidopsis bZIP transcriptionfactors	B-11958 Solid substrate fermentation of coconut coir pith for cellulase production	B-11959 Studies on preparation and viability of Phytophthora parasitica spheroplasts	B-1196 A mesh refinement scheme for finite element computations	B-11960 Identification and characterisation of Mycosphaerella graminicola secreted or surface-associated proteins with variable intragenic coding repeats	B-11961 Plant viruses spread by diffusion on ER-associated movement-protein-rafts through plasmodesmata gated by viral induced host B-1, glucanases	Next

Spheroplasts were obtained from Phytophthora parasitica mycelium by digestion with Novozym 234 using 0'35 M CaCI 2 (pH 5'7)as initial osmotic stabilizer. Spheroplast yield decreased rapidly with increasing age of the mycelium. Young mycelium gave highest yields of9'3 ±4'5 x 106 cells per Petri dish. Regeneration was investigated on synthetic and complex media in the presence of different osmotic stabilizers. Survival on solid and in liquid media was enhanced in the presence of CaCI2 in addition to mannitol as osmotic stabilizer. When spheroplasts were spread on agar containing 0'1 M CaCI 2 plus 0"4 M mannitol as osmotica the regeneration rate was 12 ± 4 x cent. After embedding in soft agarose stabilized with o-8 M mannitol spheroplast regeneration was 19 ± 6 x pent. Incubation in osmotically stabilized liquid medium resulted in a rapid loss of viability. High concentrations of CaCI 2 as osmotic stabilizer caused abnormal growth of spheroplasts which eventually gave rise to normal mycelium.

There are no comments on this title.

to post a comment.